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Endogenous fluorescence detection of neoplastic tissues could become a sensitive add-in tool for early and precise detection of different tumours in soft tissues. Endoscopic techniques and fiber-optical fluorescent probes allow achieving all organs and places in human body. New light excitation sources and sensitive detectors with high spectral resolution allow detecting low-intensity autofluorescence or to distinguish signals of specific fluorescent markers accumulated in the tumor cells. The most important moment in the current development of steady-state fluorescence technique for diagnostic applications in oncological practice is to develop appropriate databases with information about fluorescent properties of different types of pathologies for each organ and biological tissue types. One need to estimate the influence of cellular environment and state, presence of different intrinsic chromophores and pigments that could distort the emission signals detected. From diagnostic point of view, information about different tumour sub-types, state of growth, dysplastic and benign forms of lesions has to be accumulated and used for differential analysis during primary clinical observations. In this report will be presented experience on endogenous fluorescent detection based on excitation-emission matrix (EEM) development and synchronous fluorescence spectroscopy (SFS) of lower part gastrointestinal tract tumours. Malignant carcinoma lesions of colon and rectum were detected and differentiated from the normal mucosa in broad spectral range (excitation at 280-440 nm, emission at 300-800 nm) by the autofluorescent properties revealed.
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