Presentation + Paper
3 March 2022 In-vivo, in-situ, light-tunable manipulation of cells' biomechanics on a photoactive azobenzene bio-substrate
Author Affiliations +
Abstract
In-vivo, real-time study of the local and collective cellular biomechanical responses requires the fine and selective control of the cellular environment. Optical manipulation provides a suitable pathway to achieve non-contact, selective, local, temporal and spatial stimuli. The spectacular photomechanical properties of photoactive bio-substrates such as azobenzene-containing thin polymer films are a new promising strategy to achieve optically triggered local mechanical stimulation of cells. Excited cells exhibit spectacular morphological modifications and area shrinkage, which are dependent on the illumination. In this work we demonstrate that the capabilities of photomechanically active azocontaining substrates to optically stimulate cells’ mechanical response can be strongly influenced by the adhesion binding agent used to deposit the living cells on the photoactive layer. This provides a further tool for the photomechanical control of the cellular environment and of the cellular response.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Sandra Pinto, Olivier Lefebvre, Khalid Lahlil, Simon Caillaud, Jacques Peretti, Claire Smadja, Clotilde Randriamampita, Mireille Lambert, and Filippo Fabbri "In-vivo, in-situ, light-tunable manipulation of cells' biomechanics on a photoactive azobenzene bio-substrate", Proc. SPIE 11964, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX, 119640D (3 March 2022); https://doi.org/10.1117/12.2609708
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KEYWORDS
Atomic force microscopy

In vivo imaging

Polymers

Molecules

Polymer thin films

Thin films

Microscopy

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