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We report on a multimodal multiphoton microscopy (MPM) system with depth scanning. An Er-doped fiber laser with 1580 nm and 790 nm output provides the dual-wavelength multimodal capability and a shape-memory-alloy (SMA) based depth-scanning objective enables the depth scanning. Image stacks combining two-photon-excitation-fluorescence (TPEF), second-harmonic-generation (SHG), and third-harmonic-generation (THG) signals have been acquired on animal samples from the surface to over 200 μm underneath.
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Wentao Wu, Qihao Liu, Christoph Brandt, Shuo Tang, "Multimodal multiphoton microscope with depth scanning," Proc. SPIE 11965, Multiphoton Microscopy in the Biomedical Sciences XXII, 119650J (3 March 2022); https://doi.org/10.1117/12.2608365