Functionalization of a photonic crystal biosensor with modified aptamers for the detection of cardiac biomarkers

Luis Trabucco; Christian Gonzalez; Ashley Ridoutt; Ayesha Kishwar; Joshua Chaj-Ulloa; Mohammed Attia; Nevin Yazdani; Nikolay Akimov; Jing Yong Ye

doi:10.1117/12.2609898

2 March 2022 Functionalization of a photonic crystal biosensor with modified aptamers for the detection of cardiac biomarkers

Luis Trabucco, Christian Gonzalez, Ashley Ridoutt, Ayesha Kishwar, Joshua Chaj-Ulloa, Mohammed Attia, Nevin Yazdani, Nikolay Akimov, Jing Yong Ye

Author Affiliations +

Proceedings Volume 11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; 119720E (2022) https://doi.org/10.1117/12.2609898
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Label-Free detection of cardiac biomarkers has become an area of great interest with respect to point of care (POC) analysis of acute myocardial infarction and drug cardiotoxicity assays. DNA aptamers have become a potential replacement to traditional antibody detection of antigens in bioassays. In comparison to antibodies, DNA aptamers provide the advantages of lower cost, high flexibility, high batch-to-batch uniformity, stability at 37°C when immobilized on the sensor surface, and reusability with a regeneration solution. However, aptamer usage requires novel binding pathways that must be explored to ensure efficiency and consistency. Herein, a direct approach for Cardiac Troponin I (cTnI) detection was tested utilizing UV immobilization of Amino- and PolyT-modified aptamers on APTES or MPTMS modified and unmodified sensor surfaces composed of SiO₂ for a Photonic Crystal-Total Internal Reflection biosensor (PC-TIR). The detection of aptamer functionalization, and ultimately antigen detection, were monitored with a label-free bioassay system enabled by a PC-TIR sensor. Results from this study indicated that the binding pathways with the highest aptamer immobilization were: Amino modified aptamer on an APTES modified surface and PolyT modified aptamer on an MPTMS surface. Detection of the antigen was dependent on both aptamer secondary structure formation and aptamer immobilization following UV exposure.

Conference Presentation

Citation Download Citation

Luis Trabucco, Christian Gonzalez, Ashley Ridoutt, Ayesha Kishwar, Joshua Chaj-Ulloa, Mohammed Attia, Nevin Yazdani, Nikolay Akimov, and Jing Yong Ye "Functionalization of a photonic crystal biosensor with modified aptamers for the detection of cardiac biomarkers", Proc. SPIE 11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, 119720E (2 March 2022); https://doi.org/10.1117/12.2609898

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available