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Two-photon fabrication is expected to be a technique for fabricating biological tissues for regenerative medicine and drug discovery because of its capability of fabricating 3D structures on a subcellular scale. In this study, we conducted two-photon fabrication of biocompatible materials without photoinitiators. By using a visible-wavelength femtosecond pulsed laser as excitation light, two-photon polymerization is induced in deep UV absorbing moieties without the use of photo-initiators. We performed 3D micro/nanofabrication of a biocompatible hydrogel material. By using Raman spectral change, we investigated the photo-chemical process of the biocompatible upon the irradiation of visible pulsed laser light.
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Atsushi Nakayama, Yasuaki Kumamoto, Atsushi Taguchi, Katsumasa Fujita, "Photoinitiator-free micro/nano fabrication of biomaterials with nonlinear deep UV excitation," Proc. SPIE 12012, Advanced Fabrication Technologies for Micro/Nano Optics and Photonics XV, 1201203 (5 March 2022); https://doi.org/10.1117/12.2608630