Presentation + Paper
10 November 2022 Imaging of excised cochleae by micro-CT: staining, liquid embedding, and image modalities
Author Affiliations +
Abstract
Assessing the complex three-dimensional (3D) structure of the cochlea is crucial to understand fundamental aspects of signal transduction in the inner ear and is a prerequisite for the development of novel cochlear implants (CIs). Phase-contrast x-ray tomography (XPCT) offers destruction-free 3D imaging with little sample preparation, thus preserving the delicate structure of the cochlea. In this work, we present XPCT data of small-animal and human cochleae obtained at dedicated synchrotron radiation (SR) and inhouse µ-CT setups. For µ-CT we explore heavy metal stains as radiocontrast agents and show optimized phase retrieval. With the brilliant SR we achieve high contrast for unstained whole cochleae at the cellular level.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jannis J. Schaeper, M. Charles Liberman, and Tim Salditt "Imaging of excised cochleae by micro-CT: staining, liquid embedding, and image modalities", Proc. SPIE 12242, Developments in X-Ray Tomography XIV, 1224214 (10 November 2022); https://doi.org/10.1117/12.2632792
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KEYWORDS
Phase retrieval

Tomography

Sensors

Contrast transfer function

Tissues

Image quality

Laboratories

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