First demonstration of a novel nerve-targeting fluorophore in a cohort of ex vivo human tissues

Kendra A. Hebert; Logan Bateman; Dylan Parker; Jenna Nunziata; Joseph A. Paydarfar; Jennifer Hong; Darcy A. Kerr; George J. Zanazzi; Connor W. Barth; Lei Wang; Summer L. Gibbs; Eric R. Henderson

doi:10.1117/12.2650748

14 March 2023 First demonstration of a novel nerve-targeting fluorophore in a cohort of ex vivo human tissues

Kendra A. Hebert, Logan Bateman, Dylan Parker, Jenna Nunziata, Joseph A. Paydarfar, Jennifer Hong, Darcy A. Kerr, George J. Zanazzi, Connor W. Barth, Lei Wang, Summer L. Gibbs, Eric R. Henderson

Author Affiliations +

Proceedings Volume 12361, Molecular-Guided Surgery: Molecules, Devices, and Applications IX; 123610D (2023) https://doi.org/10.1117/12.2650748
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Iatrogenic nerve injury is a common complication across all surgical specialties. Better nerve visualization and identification during surgery will improve outcomes and reduce nerve injuries. The Gibbs Laboratory at Oregon Health and Science University has developed a library of near-infrared, nerve-specific fluorophores to highlight nerves intraoperatively and aid surgeons in nerve identification and visualization; the current lead agent is LGW16-03. Prior to this study, testing of LGW16-03 was restricted to animal models; therefore, it was unknown how LGW16-03 performs in human tissue. To advance LGW16-03 to clinic, we sought to test this current lead agent in ex vivo human tissues from a cohort of patients and determine if the route of administration affects LGW16-03 fluorescence contrast between nerves and adjacent background tissues (muscle and adipose). LGW16-03 was applied to ex vivo human tissue from lower limb amputations via two strategies: (1) systemic administration of the fluorophore using our first-in-kind model for fluorophore testing, and (2) topical application of the fluorophore. Results showed no statistical difference between topical and systemic administration. However, in vivo human validation of these findings is required.

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Kendra A. Hebert, Logan Bateman, Dylan Parker, Jenna Nunziata, Joseph A. Paydarfar, Jennifer Hong, Darcy A. Kerr, George J. Zanazzi, Connor W. Barth, Lei Wang, Summer L. Gibbs, and Eric R. Henderson "First demonstration of a novel nerve-targeting fluorophore in a cohort of ex vivo human tissues", Proc. SPIE 12361, Molecular-Guided Surgery: Molecules, Devices, and Applications IX, 123610D (14 March 2023); https://doi.org/10.1117/12.2650748

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