A straightforward multi-scale infrared (IR) spectroscopic characterization of anisotropic polymer nanofibers for material research and biomedical applications is presented. Polarization dependent IR spectroscopies with spatial resolutions from a few mm down to a few 10 nm (by atomic force microscopy-based infrared spectroscopy, AFM-IR) and time resolutions from the min to µs range (by infrared dual-comb polarimetry, IR-DCP) are used. Compared to AFM-IR, which measures the absorption via the photothermal expansion, and IR ellipsometry, which measures amplitudes of sand p-polarized radiation and their phase differences, IR-DCP measures separately s-and p-polarized amplitudes and phases
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