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Accurate measurements of the algal cell concentration are essential in microalgae culturing and ecological monitoring. Here, we propose a method based on optical coherence tomography (OCT) to assess green algae concentrations quantitatively and measure the depth distribution of green algae cells. The amplitude information of the complex OCT sequence was extracted to calculate the scattering coefficient. The results show that the scattering coefficient can be used to evaluate different concentrations of green algae suspensions by frequency statistics under non-contact conditions, and the scattering coefficient images can provide the depth distribution of green algae cells. This method provides an in situ, accurate and non-invasive tool for monitoring the growth of green algae and can effectively reflect the health status of water bodies.
(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.
Fan Zhang,Yuxian Zhang,Yun Tang, andJiang Zhu
"Quantification of algal cell concentration based on optical coherence tomography", Proc. SPIE 12770, Optics in Health Care and Biomedical Optics XIII, 127702R (27 November 2023); https://doi.org/10.1117/12.2687449
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Fan Zhang, Yuxian Zhang, Yun Tang, Jiang Zhu, "Quantification of algal cell concentration based on optical coherence tomography," Proc. SPIE 12770, Optics in Health Care and Biomedical Optics XIII, 127702R (27 November 2023); https://doi.org/10.1117/12.2687449