Assessment of zebrafish biomechanical properties during development using high-resolution Brillouin microscopy

Sajedeh Saeidi Fard; Andrew Lopez; Manmohan Singh; Alexander W. Schill; Salavat R. Aglyamov; Nadine Nijem; Giuliano Scarcelli; Arne Lekven; Kirill V. Larin

doi:10.1117/12.3003297

12 March 2024 Assessment of zebrafish biomechanical properties during development using high-resolution Brillouin microscopy

Sajedeh Saeidi Fard, Andrew Lopez, Manmohan Singh, Alexander W. Schill, Salavat R. Aglyamov, Nadine Nijem, Giuliano Scarcelli, Arne Lekven, Kirill V. Larin

Author Affiliations +

Proceedings Volume 12844, Optical Elastography and Tissue Biomechanics XI; 1284405 (2024) https://doi.org/10.1117/12.3003297
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Evaluating cellular biomechanics is key to understanding cellular mechanotransduction and the correlation between forces and tissue longitudinal modulus during development. Due to sub-optimal measurement techniques, this relation is poorly understood. Brillouin microscopy is a non-invasive, high-resolution, and all-optical imaging modality capable of mapping tissue longitudinal modulus. We developed a high-resolution Brillouin microscopy system to image the biomechanical characteristics of zebrafish zygotes at cell stage of 0.2 hours post fertilization (hpf), cleavage period at cell stages of 1 and 1.75 hpf, blastula period at cell stage of 3 hpf, and gastrula period at 4.3-6 hpf during development. The resulting images were able to discern the individual cells as they duplicated during development and measure cellular changes in longitudinal modulus during these stages. The results are promising and demonstrate the potential of Brillouin microscopy for revealing the biomechanical properties of the zebrafish at early developmental stages with cellular resolution.

Conference Presentation

Citation Download Citation

Sajedeh Saeidi Fard, Andrew Lopez, Manmohan Singh, Alexander W. Schill, Salavat R. Aglyamov, Nadine Nijem, Giuliano Scarcelli, Arne Lekven, and Kirill V. Larin "Assessment of zebrafish biomechanical properties during development using high-resolution Brillouin microscopy", Proc. SPIE 12844, Optical Elastography and Tissue Biomechanics XI, 1284405 (12 March 2024); https://doi.org/10.1117/12.3003297

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