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2 November 2023 Mechanisms of temperature dependence of the ratiometric signal of fluorescein
Darya P. Surzhikova, Natalia Yu. Romanova, Evgenia A. Slyusareva
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Proceedings Volume 12920, XVI International Conference on Pulsed Lasers and Laser Applications; 129200Z (2023) https://doi.org/10.1117/12.3009717
Event: XVI International Conference on Pulsed Lasers and Laser Applications, 2023, Tomsk, Russian Federation
Abstract
In the work, the priority among a number of temperature mechanisms affecting the fluorescence ratiometric signal of fluorescein in the pH range of 5.5÷7 was revealed. The kinetic model at the monoanion-dianion dissociation step with temperature-dependent constants was applied to analyze the experimental data. It was shown that the major contribution to the sensitivity of the ratiometric signal on temperature provides the perturbation of the ionic equilibrium in the ground state. The thermometric characteristics of ratiometric signal were found and the temperature resolution of 7°C was achieved. Temperature resolution can be improved up to 2°C by optimization of the experimental conditions.
(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.
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Darya P. Surzhikova, Natalia Yu. Romanova, and Evgenia A. Slyusareva "Mechanisms of temperature dependence of the ratiometric signal of fluorescein", Proc. SPIE 12920, XVI International Conference on Pulsed Lasers and Laser Applications, 129200Z (2 November 2023); https://doi.org/10.1117/12.3009717
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KEYWORDS
Quantum experiments
Quantum buffers
Quantum signals
Quantum efficiency
Quantum ground state
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