In our research, the techniques and instruments employed for detecting ultrasonic-guided waves are used to enhance rodent behavior analysis. The rodent–either a C57BL/6J or a 129 mouse–was allowed to explore and behave in an open-field arena with an aluminum plate as the floor. As the rodent moved around the open field, its voluntary and involuntary movement applied forces to the aluminum plate, leading to the generation of Lamb and Shear Horizontal (SH) waves in the plate. The generated waves contain information about the rodent’s physiology, behavior, and underlying mental states. First, this paper describes the experimental setup used for this study, emphasizing the methods adopted to facilitate a seamless measurement of reliable data. This would involve measuring the propagating waves with ultrasonic sensors and acquiring them based on the amplitude threshold criterion. Then, the work explains the tests and the techniques used to get the information in the guided waves, which can be used to infer details about the behavior, psychological state, and gait. These three aspects are explored from the tests conducted in the open field with the two strains of mice.
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