Effects of storage on stability and performance of carboxybetaine-based polymer brushes

Markéta Vrabcová; Milan Houska; Monika Spasovová; Michala Forinová; Alina Pilipenco; Ivana Matoušová Víšová; Kateřina Mrkvová; Hana Vaisocherová-Lísalová

doi:10.1117/12.3017613

20 June 2024 Effects of storage on stability and performance of carboxybetaine-based polymer brushes

Markéta Vrabcová, Milan Houska, Monika Spasovová, Michala Forinová, Alina Pilipenco, Ivana Matoušová Víšová, Kateřina Mrkvová, Hana Vaisocherová-Lísalová

Author Affiliations +

Proceedings Volume 12999, Optical Sensing and Detection VIII; 129990O (2024) https://doi.org/10.1117/12.3017613
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Zwitterionic poly(carboxybetaine) brushes have emerged as promising surface functional coatings for antifouling labelfree biosensing in complex biological fluids. This study investigates the effect of storage conditions on highly-dense poly(carboxybetaine methacrylamide) (pCBMAA), poly(sulfobetaine methacrylamide) (pSBMAA), a random copolymer of CBMAA with poly(hydroxypropyl methacrylamide) p(CBMAA-HPMAA), and terpolymer p(CBMAA-SBMAAHPMAA) brushes. Synthesized via surface-initiated atom transfer radical polymerization (SI ATRP) on gold-coated substrates, these brushes were stored in dry, water, or phosphate-buffered saline (PBS) environments at temperatures of 22 °C, 6 °C, or frozen at –20 °C for 44 days. Structural stability of the brushes was assessed using infrared reflection-absorption spectroscopy (IRRAS) and spectroscopic ellipsometry (SE), while fouling resistance and IgG antibody binding capacity were evaluated using surface plasmon resonance (SPR). Although the brushes exhibited minimal detachment from the surface, a slight decrease in swelling ratio was observed across all storage conditions. Furthermore, the storage led to a minor reduction in IgG binding capacity, with freezing at –20 °C showing a more pronounced effect in most cases. Notably, storing all tested carboxybetaine-based brushes in water or in PBS at 6 °C adversely affected fouling, a phenomenon alleviated when stored frozen at –20 °C or in a dry state.

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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Markéta Vrabcová, Milan Houska, Monika Spasovová, Michala Forinová, Alina Pilipenco, Ivana Matoušová Víšová, Kateřina Mrkvová, and Hana Vaisocherová-Lísalová "Effects of storage on stability and performance of carboxybetaine-based polymer brushes", Proc. SPIE 12999, Optical Sensing and Detection VIII, 129990O (20 June 2024); https://doi.org/10.1117/12.3017613

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