Two-photon polymerized nanopillars for surface-enhanced Raman spectroscopy

Tatevik Chalyan; Mehdi Feizpour; Heidi Ottevaere

doi:10.1117/12.3015371

20 June 2024 Two-photon polymerized nanopillars for surface-enhanced Raman spectroscopy

Tatevik Chalyan, Mehdi Feizpour, Heidi Ottevaere

Proceedings Volume 12999, Optical Sensing and Detection VIII; 129991J (2024) https://doi.org/10.1117/12.3015371
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Surface-Enhanced Raman spectroscopy (SERS) has proven its powerful ability to precisely characterize biological substances at very low concentrations down to a single molecule level without the use of a ligand or a specific biofunctionalization. SERS substrates are typically made of nanostructures coated with a thin metallic layer to produce plasmonic enhancement of the Raman scattered signal. A variety of nanofabrication technologies enable fast and economical prototyping of SERS substrates. In this work, SERS substrate fabrication based on twophoton polymerization (2PP), metrology, and biosensing characteristics are discussed. An array of polymerized nanopillars is coated by a 25 nm gold layer. Raman spectra of 1,2-bis-(4-pyridyl) ethylene (BPE) molecule at various concentrations are analyzed. The Asymmetric Least Squares (ALS) method is applied to subtract the baselines from the spectra. Next, the standard deviation (STD), enhancement factor (EF), and limit of detection (LOD) for each of the most prominent characteristic peaks of the analyte are calculated separately. The SERS substrates show up to 10⁶ Raman signal enhancement factor, comparable with commercial substrates. Moreover, the fabrication of 50 × 50 μm² SERS substrates based on the 2PP technique takes from less than a minute to 2 hours depending on the fabrication approach and aspect ratio requirements. The process is well-controlled and reproducible for reaching a uniform distribution of nanostructure arrays with an aspect ratio of up to 2.3. Finally, the SERS substrates can be used for a broader range of applications and the characterization of different molecules, like vitamins, bacteria, mycotoxins, and others.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Tatevik Chalyan, Mehdi Feizpour, and Heidi Ottevaere "Two-photon polymerized nanopillars for surface-enhanced Raman spectroscopy", Proc. SPIE 12999, Optical Sensing and Detection VIII, 129991J (20 June 2024); https://doi.org/10.1117/12.3015371

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