High resolution and fast chemical imaging using widefield optical photothermal infrared microscope

Subham Adak; Anooj Thayyil Raveendran; Jürgen Popp; Christoph Krafft

doi:10.1117/12.3016833

20 June 2024 High resolution and fast chemical imaging using widefield optical photothermal infrared microscope

Subham Adak, Anooj Thayyil Raveendran, Jürgen Popp, Christoph Krafft

Proceedings Volume 13006, Biomedical Spectroscopy, Microscopy, and Imaging III; 1300615 (2024) https://doi.org/10.1117/12.3016833
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Conference Poster

Abstract

Label-free infrared (IR) imaging reveals chemical bonds in specimens, but its limitations include a spatial resolution of approximately 5 μm. The advent of optical photothermal infrared (OPTIR) microscopy extends molecular fingerprint imaging by one order of magnitude near 500 nm. However, traditional scanning OPTIR faces a challenge of imaging speed. To improve speed and throughput, we employ pulsed visible probe light for widefield detection of transient photothermal responses induced by mid-infrared pulses. Our time-gated camera technique achieves sub-microsecond temporal resolution. We successfully imaged polystyrene beads, submicron SU8 polymer etchings, and mouse brain tissue samples using our widefield OPTIR microscope. The system excels in probe-dependent temporal and submicron spatial resolution, operating at 100 Hz over a 50 μm diameter field of view, while maintaining reasonable spectral fidelity. This enhanced widefield OPTIR microscopy promises rapid, label-free chemical imaging for biological samples and high-throughput screening applications.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Subham Adak, Anooj Thayyil Raveendran, Jürgen Popp, and Christoph Krafft "High resolution and fast chemical imaging using widefield optical photothermal infrared microscope", Proc. SPIE 13006, Biomedical Spectroscopy, Microscopy, and Imaging III, 1300615 (20 June 2024); https://doi.org/10.1117/12.3016833

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