Photosynthesis in dinoflagellates assisted by nanoparticles and their implications on cell density and metabolite production

G. Delgadillo García; S. Camacho; L. Durán

doi:10.1117/12.3028370

2 October 2024 Photosynthesis in dinoflagellates assisted by nanoparticles and their implications on cell density and metabolite production

G. Delgadillo García, S. Camacho, L. Durán

Author Affiliations +

Proceedings Volume 13110, Active Photonic Platforms (APP) 2024; 131100L (2024) https://doi.org/10.1117/12.3028370
Event: Nanoscience + Engineering, 2024, San Diego, California, United States

Conference Poster

Abstract

Lingulodinium polyedra is a microalga capable of producing toxins with antibacterial, antitumoral, and antifungal properties. Our aim was to internalize molybdenum trioxide nanoparticles (MoO₃NPs) into the microalgae Lingulodinium polyedra, leveraging the optical properties of MoO₃NPs to enhance its solar absorption and other mechanisms such as charge carriers in its photosynthetic system, thereby increasing redox reactions during the photosynthesis process. Molybdenum nanoparticles (MoNPs) were synthesized using the Laser Ablation of Solids in Liquids (LASL) method with ultrashort pulse lasers. MoNPs were characterized by UV-Vis spectroscopy, Raman spectroscopy, and Transmission Electron Microscopy (TEM). Notably, the MoNPs exhibited interesting optical properties, including an absorption peak around 800 nm, identified as a plasmonic band absorption. Using the Tauc model for semiconductors, we determined the band gap energy to be approximately 2.7 eV. We compared the evolution of MoNPs in distilled water and sterilized seawater (SSW). Over time, both environments led to the formation of MoO₃ nanoparticles (MoO₃NPs), with the MoNPs in distilled water showing a significantly different absorption spectrum compared to those in SSW. Furthermore, MoNPs were internalized into the microalgae L. polyedra. Growth curve analyses revealed that treatments with MoO₃NPs resulted in increased cell density and a longer lifespan compared to untreated algae. Additionally, through Multiple Reaction Monitoring (MRM), characteristic molecular compounds of pectenotoxins (PTXs) were detected exclusively in the dinoflagellates that internalized the MoO₃ nanoparticles.

Citation Download Citation

G. Delgadillo García, S. Camacho, and L. Durán "Photosynthesis in dinoflagellates assisted by nanoparticles and their implications on cell density and metabolite production", Proc. SPIE 13110, Active Photonic Platforms (APP) 2024, 131100L (2 October 2024); https://doi.org/10.1117/12.3028370

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