Poster
23 November 2024 All-optical 3D scanning confocal microscopy incorporating superimposed phase control
Jingrui Gong, Yue Wang, Kebin Shi
Author Affiliations +
Conference Poster
Abstract
The conventional laser confocal microscopy achieves imaging of the three-dimensional volume region by establishing the conjugate relationship between the sample space and the image space, and through the three-dimensional motion of the sample. However, the movement of the sample stage not only reduces the imaging speed and introduces motion artifacts, but also limits the imaging samples to forms such as slices that are compatible with the sample holder, precluding in vivo three-dimensional imaging. In this study, by introducing phase control under end-to-end closed-loop control, precise and aberration-free all-optical three-dimensional high-speed scanning with high numerical aperture excitation is achieved. Without the need for sample movement, two-dimensional imaging at a kHz speed with 300 nm lateral resolution and a three-dimensional volume imaging speed in the hundreds of Hertz are realized, enabling in vivo real-time high-resolution three-dimensional imaging of research objects such as living mice in various life states.
© (2024) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jingrui Gong, Yue Wang, and Kebin Shi "All-optical 3D scanning confocal microscopy incorporating superimposed phase control", Proc. SPIE 13238, Advanced Optical Imaging Technologies VII, 132380W (23 November 2024); https://doi.org/10.1117/12.3036418
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KEYWORDS
Biological imaging

Biological samples

3D image processing

Confocal microscopy

3D scanning

In vivo imaging

Image resolution

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