Effectiveness of photosensitive dye during uptake and redistribution

Xiao-Yan He M.D.; Steven L. Jacques; Gary Gofstein

doi:10.1117/12.60943

1 June 1992 Effectiveness of photosensitive dye during uptake and redistribution

Xiao-Yan He M.D., Steven L. Jacques, Gary Gofstein

Proceedings Volume 1645, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy; (1992) https://doi.org/10.1117/12.60943
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

The location of photosensitive dye within a cell will affect the efficacy of photodynamic therapy (PDT). This report demonstrates that during the first 3 hours of dye (Photofrin porfimer) uptake from a liquid medium, the dye is diffusely distributed within the cell. After 24 hours of dye uptake, the dye is localized in specific sites within the cell. Fluorescence spectroscopy demonstrated that the 3-hour dye's emission peak near 635 nm was slightly blue shifted for the localized 24-hour dye. This paper demonstrates that the diffuse dye (at 3 hours) is more effective for PDT than the localized dye (at 24 hours). The amount of diffuse dye within the cell that is required to achieve an LD₅₀ (50% lethal dose) is 1.5-fold to 4.5- fold less than the amount of localized dye required, using the same light exposure. The range of 1.5 - 4.5-fold refers to the results for four cell lines.

Citation Download Citation

Xiao-Yan He M.D., Steven L. Jacques, and Gary Gofstein "Effectiveness of photosensitive dye during uptake and redistribution", Proc. SPIE 1645, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy, (1 June 1992); https://doi.org/10.1117/12.60943

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