Characterization of imaging from a three-dimensional optical microscope

David F. Abbott; Keith A. Nugent

doi:10.1117/12.59553

26 June 1992 Characterization of imaging from a three-dimensional optical microscope

David F. Abbott, Keith A. Nugent

Proceedings Volume 1660, Biomedical Image Processing and Three-Dimensional Microscopy; (1992) https://doi.org/10.1117/12.59553
Event: SPIE/IS&T 1992 Symposium on Electronic Imaging: Science and Technology, 1992, San Jose, CA, United States

Abstract

Characterization of a three-dimensional optical microscope is essential before any image processing can be performed to deblur optical section images. The conventional approach has been to measure the entire 3-D point spread function (PSF) of the system. In this paper, we discuss a new approach which requires only three 2-D defocused PSFs. With these measurements, it is found to be possible to determine the spherical aberration in a system. Once the system is characterized, 3-D image processing can proceed using the 3-D point spread function. A sampling theorem has been proposed, and is reviewed here, which provides conditions for the validity of linear image processing. We discuss and present some of the ramifications of this result on simulated images using measured point spread functions.

Citation Download Citation

David F. Abbott and Keith A. Nugent "Characterization of imaging from a three-dimensional optical microscope", Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); https://doi.org/10.1117/12.59553

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