Role of x-ray-induced damage in biological micro-imaging

Richard A. London; James E. Trebes; Chris J. Jacobsen

doi:10.1117/12.138749

13 January 1993 Role of x-ray-induced damage in biological micro-imaging

Richard A. London, James E. Trebes, Chris J. Jacobsen

Proceedings Volume 1741, Soft X-Ray Microscopy; (1993) https://doi.org/10.1117/12.138749
Event: San Diego '92, 1992, San Diego, CA, United States

Abstract

Interactions of x rays with a sample are studied to determine the optimal wavelength, source energy, and exposure time for microcopy and holography. The optimal wavelength is influenced by two criteria: minimizing the required source energy and minimizing the absorbed dose and subsequent damage to the sample. The use of heavy element labels, such as colloidal gold, can significantly reduce the energy and dose. Limits to the exposure time due to natural motions, x-ray induced chemical damage, heat build-up, and hydrodynamic expansion are discussed.

Citation Download Citation

Richard A. London, James E. Trebes, and Chris J. Jacobsen "Role of x-ray-induced damage in biological micro-imaging", Proc. SPIE 1741, Soft X-Ray Microscopy, (13 January 1993); https://doi.org/10.1117/12.138749

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