Optical triple sensor for measuring pH, oxygen, and carbon dioxide in bioreactors

Bernhard H. Weigl; Andrej Holobar; Wolfgang Trettnak; Ingo Klimant; H. Kraus; Paul O'Leary; Otto S. Wolfbeis

doi:10.1117/12.143538

30 April 1993 Optical triple sensor for measuring pH, oxygen, and carbon dioxide in bioreactors

Bernhard H. Weigl, Andrej Holobar, Wolfgang Trettnak, Ingo Klimant, H. Kraus, Paul O'Leary, Otto S. Wolfbeis

Proceedings Volume 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV; (1993) https://doi.org/10.1117/12.143538
Event: Fibers '92, 1992, Boston, MA, United States

Abstract

A triple sensor unit consisting of opto-chemical sensors for measurement of pH, oxygen, and carbon dioxide is presented. The pH sensor and the CO₂ sensor are based on the color change of a pH-sensitive dye immobilized on a polymeric support. The resulting changes in absorption are monitored through optical fibers at one or two analytical wavelengths. The oxygen sensor is based on the quenching of the fluorescence of a metalorganic dye. The operation principle and the performance of all three sensors are described thoroughly with respect to their application in bioreactors. All three sensors are fully LED compatible. The chemical and mechanical stability, especially against common sterilization methods, are described in some detail. A calibration and measurement software comprising fit routines for the sensors and a mathematical treatment of the results are presented as well.

Citation Download Citation

Bernhard H. Weigl, Andrej Holobar, Wolfgang Trettnak, Ingo Klimant, H. Kraus, Paul O'Leary, and Otto S. Wolfbeis "Optical triple sensor for measuring pH, oxygen, and carbon dioxide in bioreactors", Proc. SPIE 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV, (30 April 1993); https://doi.org/10.1117/12.143538

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