Development and characteristics of a new picosecond fluorescence lifetime system

Motoyuki Watanabe; Musubu Koishi; Paul W. Roehrenbeck

doi:10.1117/12.144707

18 May 1993 Development and characteristics of a new picosecond fluorescence lifetime system

Motoyuki Watanabe, Musubu Koishi, Paul W. Roehrenbeck

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144707
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

A new time-resolved fluorescence measurement system has been developed. A compact streakscope is used as a detector, and the combination of a spectrograph and a streakscope enables the instrument to measure the fluorescence lifetime and spectrum simultaneously using the single photon counting method with a time resolution of 15 ps (5 ps with fitting software). The dynamic range is achieved up to 105. The instrument response function (IRF) of a streakscope is nearly Gaussian, and it has no tail and no `after pulses' which are observed at the IRF of a photomultiplier used in the time correlated single photon counting system (TCPC). So, a short lifetime of several ten picoseconds can be measured with accuracy. In addition, two dimensional (time and wavelength) single photon counting can be performed without scanning the wavelength, so time resolved spectrum can be measured in a short time with a high S/N ratio.

Citation Download Citation

Motoyuki Watanabe, Musubu Koishi, and Paul W. Roehrenbeck "Development and characteristics of a new picosecond fluorescence lifetime system", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144707

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