Quantitative determination of conformational order in biomembranes: from aqueous monolayers to living cells

Richard Mendelsohn

doi:10.1117/12.166778

31 January 1994 Quantitative determination of conformational order in biomembranes: from aqueous monolayers to living cells

Richard Mendelsohn

Author Affiliations +

Proceedings Volume 2089, 9th International Conference on Fourier Transform Spectroscopy; (1994) https://doi.org/10.1117/12.166778
Event: Fourier Transform Spectroscopy: Ninth International Conference, 1993, Calgary, Canada

Abstract

Two regions of the IR spectrum of phospholipid molecules have been utilized to monitor acyl chain conformational order in model biomembranes of increasing complexity from one component systems to living cells. The rocking modes of CD₂ groups synthetically incorporated into the acyl chains provide a measure of % gauche roamer formation as a function of chain position from the interfacial region to the bilayer center. In addition, the CH₂ wagging vibrations in disordered phases provide a means of identifying the particular two- and three-bond conformational states that dominate the disordering process, while the band progressions that result from the wagging of coupled CH₂ oscillators in ordered states monitor the fraction of all-trans conformations.

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Richard Mendelsohn "Quantitative determination of conformational order in biomembranes: from aqueous monolayers to living cells", Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); https://doi.org/10.1117/12.166778

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