Ultrasensitive fluorescence detection and maximum likelihood method

Joerg Enderlein

doi:10.1117/12.180805

21 July 1994 Ultrasensitive fluorescence detection and maximum likelihood method

Joerg Enderlein

Proceedings Volume 2136, Biochemical Diagnostic Instrumentation; (1994) https://doi.org/10.1117/12.180805
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

The maximum likelihood criterion is shown to be a powerful method for analyzing fluorescence detection data with small signal to noise ratios. Probability studies of the maximum likelihood criterion for supposed detection experiments of very small amounts of fluorescing molecules, down to a single molecule, are presented. In these studies the photokinetics of the molecules, their flow and diffusion, and the laser-beam geometry are taken into account. The efficiency of time-integrated and time-correlated single-photon counting methods are studied and compared.

Citation Download Citation

Joerg Enderlein "Ultrasensitive fluorescence detection and maximum likelihood method", Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); https://doi.org/10.1117/12.180805

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