Near-IR biosensor for evanescent wave immunoassays

Joel P. Golden; Lisa C. Shriver-Lake; Narasimhachari Narayanan; Gabor Patonay; Frances S. Ligler

doi:10.1117/12.181363

27 July 1994 Near-IR biosensor for evanescent wave immunoassays

Joel P. Golden, Lisa C. Shriver-Lake, Narasimhachari Narayanan, Gabor Patonay, Frances S. Ligler

Proceedings Volume 2138, Longer Wavelength Lasers and Applications; (1994) https://doi.org/10.1117/12.181363
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Based upon a biosensor design which utilizes standard fluorescent dyes (FITC, TRITC), a new device has been developed which incorporates a laser diode light source to excite novel near infrared (NIR) dyes. The purpose of switching to the NIR regime is to decrease the background fluorescence of biological samples and to decrease the size and power requirements of the biosensor. New dyes which fluoresce in the NIR have been conjugated to protein antigen and immunoassays performed. Assay results using excitation at 780 nm are shown.

Citation Download Citation

Joel P. Golden, Lisa C. Shriver-Lake, Narasimhachari Narayanan, Gabor Patonay, and Frances S. Ligler "Near-IR biosensor for evanescent wave immunoassays", Proc. SPIE 2138, Longer Wavelength Lasers and Applications, (27 July 1994); https://doi.org/10.1117/12.181363

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