Chondrocytes provide a model for in-situ confocal microscopy and 3D reconstructions

Michelle S. Hirsch; Kathy K. H. Svoboda

doi:10.1117/12.172089

4 April 1994 Chondrocytes provide a model for in-situ confocal microscopy and 3D reconstructions

Michelle S. Hirsch, Kathy K. H. Svoboda

Proceedings Volume 2184, Three-Dimensional Microscopy: Image Acquisition and Processing; (1994) https://doi.org/10.1117/12.172089
Event: IS&T/SPIE 1994 International Symposium on Electronic Imaging: Science and Technology, 1994, San Jose, CA, United States

Abstract

Hyaline cartilage is composed of chondrocytes that reside in lacunae surrounded by extracellular matrix molecules. Microscopic and histochemical features of cartilage have been studied with many techniques. Many of these techniques can be time consuming and may alter natural cartilage characteristics. In addition, the orientation and order of sectioned tissue must be maintained to create 3D reconstructions. We show that confocal laser scanning microscopy may replace traditional methods for studying cartilage.

Citation Download Citation

Michelle S. Hirsch and Kathy K. H. Svoboda "Chondrocytes provide a model for in-situ confocal microscopy and 3D reconstructions", Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); https://doi.org/10.1117/12.172089

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