Estimation of the parameters of dye distributions by optical probing

Gerd C. Beck; Alwin Kienle; Rudolf W. Steiner

doi:10.1117/12.200825

31 January 1995 Estimation of the parameters of dye distributions by optical probing

Gerd C. Beck, Alwin Kienle, Rudolf W. Steiner

Proceedings Volume 2326, Photon Transport in Highly Scattering Tissue; (1995) https://doi.org/10.1117/12.200825
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

The observable parameters remittance, transmittance and fluorescence emission of layered systems are determined from Monte Carlo simulations and the diffusion approximation. We calculate the effect of additional absorption caused by a fluorescing dye, depending on dye- bearing layer thickness. The results of the simulations are compared to the diffusion approximation, establishing a range of accuracy of the diffusion approximation. We show that, provided the basic optical properties of the system are known, additional absorption and layer thickness can be calculated from non-invasive measurements of remittance and fluorescence emission. We give an estimation of the effect of experimental uncertainty on the results for dye absorption and layer thickness.

Citation Download Citation

Gerd C. Beck, Alwin Kienle, and Rudolf W. Steiner "Estimation of the parameters of dye distributions by optical probing", Proc. SPIE 2326, Photon Transport in Highly Scattering Tissue, (31 January 1995); https://doi.org/10.1117/12.200825

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