Detection of singlet oxygen yield from new photosensitizers using luminol

Harumasa Sakai; Hugo Oppelaar; Paul Baas; Nico Van Zandwijk; Fiona A. Stewart

doi:10.1117/12.203354

1 March 1995 Detection of singlet oxygen yield from new photosensitizers using luminol

Harumasa Sakai, Hugo Oppelaar, Paul Baas, Nico Van Zandwijk, Fiona A. Stewart

Proceedings Volume 2371, 5th International Photodynamic Association Biennial Meeting; (1995) https://doi.org/10.1117/12.203354
Event: Fifth International Photodynamic Association Biennial Meeting, 1994, Amelia Island, FL, United States

Abstract

For the application of photodynamic therapy and diagnosis many different photosensitizers have been developed. It is important to compare these photosensitizers for their activity. It is generally accepted that the most important mechanism of cell killing is via the production of singlet oxygen. We therefore performed basic studies to detect singlet oxygen using a luminol reaction. The relative singlet oxygen yields from 4 photosensitizers (Photofrin, ATX-S10, mTHPC and NPe6) were measured by the detection of luminol chemiluminescence at 445 nm wavelength in Menzel's buffer solution at pH 10.5. NPe6, ATX-S10 and mTHPC all showed singlet oxygen productive abilities. These photosensitizers are new promising photosensitizers. These results show a possibility of comparison of each photosensitizer.

Citation Download Citation

Harumasa Sakai, Hugo Oppelaar, Paul Baas, Nico Van Zandwijk, and Fiona A. Stewart "Detection of singlet oxygen yield from new photosensitizers using luminol", Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); https://doi.org/10.1117/12.203354

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