Polarization contrast in scanning microscopy

Tony Wilson; Rimas Juskaitis

doi:10.1117/12.205323

23 March 1995 Polarization contrast in scanning microscopy

Tony Wilson, Rimas Juskaitis

Proceedings Volume 2412, Three-Dimensional Microscopy: Image Acquisition and Processing II; (1995) https://doi.org/10.1117/12.205323
Event: IS&T/SPIE's Symposium on Electronic Imaging: Science and Technology, 1995, San Jose, CA, United States

Abstract

We discuss the differences between polarization contrast imaging in conventional and confocal systems and show that because of the fundamentally different imaging properties of the two systems, the extinction coefficient is non-zero in a conventional system even with perfect polars. We then discuss the axial response of high numerical aperture systems and show that a polarization effect leads to an asymmetric response when the objective lens numerical aperture is greater than the refractive index of the specimen.

Citation Download Citation

Tony Wilson and Rimas Juskaitis "Polarization contrast in scanning microscopy", Proc. SPIE 2412, Three-Dimensional Microscopy: Image Acquisition and Processing II, (23 March 1995); https://doi.org/10.1117/12.205323

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