Effect of apodization on axial resolution with a high-aperture objective

Min Gu

doi:10.1117/12.237494

10 April 1996 Effect of apodization on axial resolution with a high-aperture objective

Min Gu

Proceedings Volume 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III; (1996) https://doi.org/10.1117/12.237494
Event: Electronic Imaging: Science and Technology, 1996, San Jose, CA, United States

Abstract

We investigate the effects of the apodization functions of a high-aperture objective on axial resolution in confocal bright-field microscopy. It is found that the measured apodization function for a commercial objective deviates significantly from the function predicted under the sine condition, which is usually assumed to be satisfied by a commercial objective. This result may explain the long-time discrepancy of the axial resolution between theoretical prediction and measured results, and also the observation of smoothly oscillating side peaks in the axial response when the objective is operated at an incorrect tube length. This finding is of importance when one tries to compensate for the spherical aberrations, with a certain accuracy, by the alteration of tube length of the objective. The deviation of apodization of a practical objective from the sine condition may be also responsible for the observation of the broadened axial response in confocal fluorescence microscopy including 4Pi confocal systems.

Citation Download Citation

Min Gu "Effect of apodization on axial resolution with a high-aperture objective", Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); https://doi.org/10.1117/12.237494

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