Influence of second harmonic alexandrite-laser radiation on bacterial growth

Thomas Hennig; Peter Rechmann D.D.S.; Thomas Pecnik; Hans-Peter Heinz; Ulrich Hadding

doi:10.1117/12.238783

23 April 1996 Influence of second harmonic alexandrite-laser radiation on bacterial growth

Thomas Hennig, Peter Rechmann D.D.S., Thomas Pecnik, Hans-Peter Heinz, Ulrich Hadding

Author Affiliations +

Proceedings Volume 2672, Lasers in Dentistry II; (1996) https://doi.org/10.1117/12.238783
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

The aim of this study was to investigate the impact of 2nd harmonic Alexandrite-laser radiation (wavelength 380 nm, pulse duration 100 ns) on the growth of bacteria. Bacteriodes vulgatus was chosen as typical and easy to handle anaerobic germ. Escherichia coli wild-type was chosen as a control germ, which is well investigated by many other groups at a wide range of different irradiation conditions. All germs were cultured on agar plates, suspended and irradiated in an optical transparent irradiated medium, and afterwards plated on agar again. An optical transparent irradiation medium minimizes photothermal and photochemical side effects onto the bacteria, which otherwise would be induced by absorption of the laser radiation in an optical opaque culture medium. Imaging of a quartz fiber surface onto the bacterial suspension guaranteed homogeneous irradiation of the sample. Different total energies were achieved by different numbers of pulses. Inhibition of bacterial growth could be observed for both germs. The effectiveness is depending on the type of bacteria and on total energy applied.

Citation Download Citation

Thomas Hennig, Peter Rechmann D.D.S., Thomas Pecnik, Hans-Peter Heinz, and Ulrich Hadding "Influence of second harmonic alexandrite-laser radiation on bacterial growth", Proc. SPIE 2672, Lasers in Dentistry II, (23 April 1996); https://doi.org/10.1117/12.238783

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