Imaging liquid crystalline mesophases in vivo and in vitro: measuring molecular birefringence and order parameter in liquid crystals

Yu-Ming Zhou; Richard H. Newton; J. Haffegee; Jacki Y. Brown; Stephen Ross; John P. Bolton; Mae-Wan Ho

doi:10.1117/12.260804

6 December 1996 Imaging liquid crystalline mesophases in vivo and in vitro: measuring molecular birefringence and order parameter in liquid crystals

Yu-Ming Zhou, Richard H. Newton, J. Haffegee, Jacki Y. Brown, Stephen Ross, John P. Bolton, Mae-Wan Ho

Author Affiliations +

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260804
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

A recent technique optimizing the detection of small birefringences typical of biological liquid crystals has been described elsewhere. Here, we derive a liner relationship between color intensity, molecular birefringence and degree of phase alignment, based on which, a quantitative image analysis is developed. The image analysis is used to define the dynamics of the phase transition-like increase in color intensity accompanying the condensation of the body-wall musculature in the maturing Drosophila larva, to map the orientation of the collagen fibers in the intervertebral disc, and to investigate mesophases of pork skin collagen assembled in vitro.

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Yu-Ming Zhou, Richard H. Newton, J. Haffegee, Jacki Y. Brown, Stephen Ross, John P. Bolton, and Mae-Wan Ho "Imaging liquid crystalline mesophases in vivo and in vitro: measuring molecular birefringence and order parameter in liquid crystals", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260804

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