Lifetime identification of single molecules in aqueous solution

Christoph Zander; Markus Sauer; Karl-Heinz Drexhage; Juergen M. Wolfrum; Leif Brand; C. Eggeling; Claus A. M. Seidel

doi:10.1117/12.273566

7 May 1997 Lifetime identification of single molecules in aqueous solution

Christoph Zander, Markus Sauer, Karl-Heinz Drexhage, Juergen M. Wolfrum, Leif Brand, C. Eggeling, Claus A. M. Seidel

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273566
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Photon bursts of single rhodamine 6G and rhodamine B molecules in aqueous solution were studied by excitation with a frequency-doubled titanium: sapphire laser. Multichannel scalar traces, fluorescence correlation functions and fluorescence decays determined by time- correlated single-photon counting have been measured simultaneously. The time-resolved fluorescence signals were analyzed with a maximum likelihood estimator. With the setup described it is possible to distinguish single dye molecules of different kind via their characteristic fluorescence lifetimes of 1.79 +/- 0.33 ns for rhodamine B-zwitterion and 3.79 +/- 0.38 ns for rhodamine 6G.

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Christoph Zander, Markus Sauer, Karl-Heinz Drexhage, Juergen M. Wolfrum, Leif Brand, C. Eggeling, and Claus A. M. Seidel "Lifetime identification of single molecules in aqueous solution", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273566

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