Paper
10 April 1997 Three-dimensional imaging through millimeter-thick tissue specimens
Michael G. Jones, Michael Halliwell, David R. Bull, Jack D. Davies, Catherine N. Chinyama, Peter N. T. Wells
Author Affiliations +
Proceedings Volume 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV; (1997) https://doi.org/10.1117/12.271263
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States
Abstract
A 780 nm stage-scanned confocal transmission microscope using compact disk (CD) player optics has been constructed and used to study test objects (including scattering effects) and breast tissue specimens (25-1500 micrometer thick). Confocal alignment in transmission is achieved using the CD optical mechanism moving coils to scan the objective lens until intensity peaks; the specimen is present but motionless. Intensity variation as a 3D function of mis- alignment of the two foci may allow estimation of optic deterioration caused by the specimen; appropriate confocal apertures and sample spacings can then be selected. Experimental measurements have been supplemented by 2D Monte-Carlo modeling of photon transport for a scattering- limited confocal transmission system. Modeling has characterized: transmission as a function of focal depth in a slab; contrast produced by a range of embedded spherical target radii; and optical mis-alignment. Poor dye perfusion within thick stained specimens results in little visible internal structure. This may be improved using partially phase dependent imaging (approximately equal to split- detector), sensitive to specimen refractive index variations.
© (1997) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Michael G. Jones, Michael Halliwell, David R. Bull, Jack D. Davies, Catherine N. Chinyama, and Peter N. T. Wells "Three-dimensional imaging through millimeter-thick tissue specimens", Proc. SPIE 2984, Three-Dimensional Microscopy: Image Acquisition and Processing IV, (10 April 1997); https://doi.org/10.1117/12.271263
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KEYWORDS
Scattering

Confocal microscopy

Tissues

Tissue optics

Monte Carlo methods

Microscopes

Light scattering

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