Action of low-power laser irradiation on the proliferation of human gingival fibroblasts in vitro

Luciana Almeida-Lopes D.D.S.; Marcia M. M. Jaeger; Aldo Brugnera Jr.; Josepa Rigau

doi:10.1117/12.306022

22 April 1998 Action of low-power laser irradiation on the proliferation of human gingival fibroblasts in vitro

Luciana Almeida-Lopes D.D.S., Marcia M. M. Jaeger, Aldo Brugnera Jr., Josepa Rigau

Author Affiliations +

Proceedings Volume 3248, Lasers in Dentistry IV; (1998) https://doi.org/10.1117/12.306022
Event: BiOS '98 International Biomedical Optics Symposium, 1998, San Jose, CA, United States

Abstract

The low level power laser has been used in dental treatments aiming to improve tissue healing. An in vitro study was performed to analyze the laser influence on gingival fibroblast. A human gingival fibroblast culture (LMF) was produced in DME medium with 10% bovine fetal serum (BFS) cells (LMF) were allocated in Petri plates and cultured in different SFB concentrations (0%, 5% e 10%). After 48 hours the plates were divided in 9 groups: 3 control: 3 irradiated by 635 nm laser; and 3 irradiated by 780 nm laser. The cultured cells received 4 applications, in 12 hours intervals, with energy dosage of 2 joules for each plate, by means of a punctual technique. The growth curves showed that the growth levels were lower in low BFS concentrations. The irradiation with laser accelerated the growth rate in all groups. Additionally, the number of cells developed in low BFS concentration (5%) and irradiated was similar to the number of control cells developed in ideal conditions (10% BFS). There was no statistically significant differences between the effects of the two types of laser studied.

Citation Download Citation

Luciana Almeida-Lopes D.D.S., Marcia M. M. Jaeger, Aldo Brugnera Jr., and Josepa Rigau "Action of low-power laser irradiation on the proliferation of human gingival fibroblasts in vitro", Proc. SPIE 3248, Lasers in Dentistry IV, (22 April 1998); https://doi.org/10.1117/12.306022

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