Intracellular gradients of free calcium visualized in sensory and neuronal cells by a high-performance fluorescence imaging system

Alberto Colavita; Gabriele Capello; Razaq B. Ijaduola; Antonio Cunei; Laura Lagostena; Marco Canepari; Fabio Mammano

doi:10.1117/12.349212

1 June 1999 Intracellular gradients of free calcium visualized in sensory and neuronal cells by a high-performance fluorescence imaging system

Alberto Colavita, Gabriele Capello, Razaq B. Ijaduola, Antonio Cunei, Laura Lagostena, Marco Canepari, Fabio Mammano

Author Affiliations +

Proceedings Volume 3604, Optical Diagnostics of Living Cells II; (1999) https://doi.org/10.1117/12.349212
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

This paper describes results obtained with a novel imaging system based on a fast CCD device. Sensor's output was digitized at 12 bit/pixel by customized electronics, reaching acquisition rates as high as 800 frames/s with a full-frame resolution of 128 X 128 pixels. The software developed for the project permitted the sequential capture of thousands of images directly to host PC RAM without frame loss even at the maximum readout rate (16 MHz). It is shown that the high spatio-temporal resolution of this apparatus is of value when investigating the time-course of rapid intracellular Ca²⁺ fluorescence transients, particularly those associated with neuronal action potentials near physiological temperature.

Citation Download Citation

Alberto Colavita, Gabriele Capello, Razaq B. Ijaduola, Antonio Cunei, Laura Lagostena, Marco Canepari, and Fabio Mammano "Intracellular gradients of free calcium visualized in sensory and neuronal cells by a high-performance fluorescence imaging system", Proc. SPIE 3604, Optical Diagnostics of Living Cells II, (1 June 1999); https://doi.org/10.1117/12.349212

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available