Functional imaging of single cells with photothermal microscopy

Dmitry Lapotko

doi:10.1117/12.386330

19 May 2000 Functional imaging of single cells with photothermal microscopy

Dmitry Lapotko

Proceedings Volume 3916, Biomedical Optoacoustics; (2000) https://doi.org/10.1117/12.386330
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Photothermal technique for light microscopy is reported as the new tool for quantitative analysis of single living cell structural and functional properties. Probe laser illumination with phase contrast method allows to visualize thermal field being induced in cell due to absorption of pump laser radiation. In our set up the cell is pumped by the pulsed laser at 532 nm, 10 ns, 0.01-0.4 mJ. The source of probe beam is a pulsed dye laser which forms cell image. Fully automated image cytometer with acquisition rate 1cell/s is described. An acquired cell image is considered as spatially and temporally resolved cell response to non- specific load being induced with a pump laser. Thus we introduce photothermal image cytometry which may be applied for investigation of mechanisms of photodamage, single cell dosimetry, cell functions and structure and thermal phenomena at cell level.

Citation Download Citation

Dmitry Lapotko "Functional imaging of single cells with photothermal microscopy", Proc. SPIE 3916, Biomedical Optoacoustics, (19 May 2000); https://doi.org/10.1117/12.386330

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