Multispectral excitation of time-resolved fluorescence of biological compounds: variation of fluorescence lifetime with excitation and emission wavelengths

Jean-Michel I. Maarek; Sungheon Kim

doi:10.1117/12.426734

10 May 2001 Multispectral excitation of time-resolved fluorescence of biological compounds: variation of fluorescence lifetime with excitation and emission wavelengths

Jean-Michel I. Maarek, Sungheon Kim

Author Affiliations +

Proceedings Volume 4252, Advances in Fluorescence Sensing Technology V; (2001) https://doi.org/10.1117/12.426734
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

We investigated the time-resolved fluorescence spectra of tissue structural proteins elastin and collagen I for different excitation wavelengths to assess the usefulness of multispectral excitation for characterization of fluorescent structural proteins in tissue. Laser excitation pulses (4 ns FWHM) at wavelengths between 337 nm and 422 nm were directed to the samples with a multifiber fluorescence probe. The fluorescence emission was measured with a photomultiplier and deconvoluted from the emission pulse with an algorithm based on the Laguerre expansion of kernels technique. A multiexponential approximation of the intrinsic fluorescence decay was computed using a global approach in which the decay constants were held fixed across wavelengths of emission.

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Jean-Michel I. Maarek and Sungheon Kim "Multispectral excitation of time-resolved fluorescence of biological compounds: variation of fluorescence lifetime with excitation and emission wavelengths", Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); https://doi.org/10.1117/12.426734

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