Fabrication of polyurethane molecular stamps for the synthesis of DNA microarray

Zhengchun Liu; Quanguo He; Pengfeng Xiao; Nongyao He; Zuhong Lu; Liang Bo

doi:10.1117/12.444752

15 October 2001 Fabrication of polyurethane molecular stamps for the synthesis of DNA microarray

Zhengchun Liu, Quanguo He, Pengfeng Xiao, Nongyao He, Zuhong Lu, Liang Bo

Proceedings Volume 4601, Micromachining and Microfabrication Process Technology and Devices; (2001) https://doi.org/10.1117/12.444752
Event: International Symposium on Optoelectonics and Microelectronics, 2001, Nanjing, China

Abstract

Polyurethane based on polypropylene glycol (PPG) and Toluene diisocyanate (TDI) using 3,3'-dichloride-4,4'- methylenedianiline (MOCA) as the crosslinker is presented for the first time to fabricate molecular stamps (PU stamps) for the synthesis of DNA microarray with contact procedure. The predictability of the process is achieved by utilizing commercially available starting materials. SEM analysis of the morphology of PU stamps and master showed that PU elastometer could replicate subtly the motherboard's patterns with high fidelity. It was proved from the contact angle measurement that PU stamps surface has good affinity with acetonitrile, which guarantee the well-distribution of DNA monomers on patterned stamps. Laser confocal fluorescence microscopy images of oligonucleotide arrays confirmed polyurethane is an excellent material for molecular stamps.

Citation Download Citation

Zhengchun Liu, Quanguo He, Pengfeng Xiao, Nongyao He, Zuhong Lu, and Liang Bo "Fabrication of polyurethane molecular stamps for the synthesis of DNA microarray", Proc. SPIE 4601, Micromachining and Microfabrication Process Technology and Devices, (15 October 2001); https://doi.org/10.1117/12.444752

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