Cell dynamics with a novel phase referenced low-coherence interferometer with sub-wavelength and sub-hertz sensitivity

Changhuei Yang; Adam Wax; Kamran Badizadegan; Ramachandra R. Dasari; Michael S. Feld

doi:10.1117/12.470484

14 June 2002 Cell dynamics with a novel phase referenced low-coherence interferometer with sub-wavelength and sub-hertz sensitivity

Changhuei Yang, Adam Wax, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Author Affiliations +

Proceedings Volume 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI; (2002) https://doi.org/10.1117/12.470484
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

We report the use of a highly sensitive phase based motion measurement technique to study the correlation of cellular metabolic rate with cellular motions. The technique is based on a modified Michelson interferometer with a composite laser beam of 1550 nm low coherence light and 775 nm CW light. In this system, motional artifacts from vibrations in the interferometer are completely eliminated. We demonstrate that the system is sensitive to motions as small as 3.6 nm and velocities as small as 1 nm/s. Using the system, we show that the cellular motions are strongly dependent on the ambient temperature. We observe that the dependency does not conform to Brownian motion predictions but instead appears to correlate with the optical ambient temperature that the cells have evolved to operate in.

Citation Download Citation

Changhuei Yang, Adam Wax, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld "Cell dynamics with a novel phase referenced low-coherence interferometer with sub-wavelength and sub-hertz sensitivity", Proc. SPIE 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI, (14 June 2002); https://doi.org/10.1117/12.470484

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