Determining quantitative immunophenotypes and evaluating their implications

Douglas Redelman; Dorothy Hudig; Dave Berner; Linda M. Castell; Don Roberts; Wayne Ensign

doi:10.1117/12.468347

28 May 2002 Determining quantitative immunophenotypes and evaluating their implications

Douglas Redelman, Dorothy Hudig, Dave Berner, Linda M. Castell, Don Roberts, Wayne Ensign

Proceedings Volume 4622, Optical Diagnostics of Living Cells V; (2002) https://doi.org/10.1117/12.468347
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Quantitative immunophenotypes varied widely among > 100 healthy young males but were maintained at characteristic levels within individuals. The initial results (SPIE Proceedings 4260:226) that examined cell numbers and the quantitative expression of adhesion and lineage-specific molecules, e.g., CD2 and CD14, have now been confirmed and extended to include the quantitative expression of inducible molecules such as HLA-DR and perforin (Pf). Some properties, such as the ratio of T helper (Th) to T cytotoxic/suppressor (Tc/s) cells, are known to be genetically determined. Other properties, e.g., the T:B cell ratio, the amount of CD19 per B cell, etc., behaved similarly and may also be inherited traits. Since some patterns observed in these healthy individuals resembled those found in pathological situations we tested whether the patterns could be associated with the occurrence of disease. The current studies shows that there were associations between quantitative immunophenotypes and the subsequent incidence and severity of disease. For example, individuals with characteristically low levels of HLA-DR or B cells or reduced numbers of Pf+ Tc/s cells had more frequent and/or more severe upper respiratory infections. Quantitative immunophenotypes will be more widely measured if the necessary standards are available and if appropriate procedures are made more accessible.

Citation Download Citation

Douglas Redelman, Dorothy Hudig, Dave Berner, Linda M. Castell, Don Roberts, and Wayne Ensign "Determining quantitative immunophenotypes and evaluating their implications", Proc. SPIE 4622, Optical Diagnostics of Living Cells V, (28 May 2002); https://doi.org/10.1117/12.468347

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