Alternative chromophores for use in light-activated surgical adhesives: optimization of parameters for tensile strength and thermal damage profile

Grant T. Hoffman; Brian D. Byrd; Eric C. Soller; Douglas L. Heintzelman M.D.; Karen M. McNally-Heintzelman

doi:10.1117/12.476389

12 September 2003 Alternative chromophores for use in light-activated surgical adhesives: optimization of parameters for tensile strength and thermal damage profile

Grant T. Hoffman, Brian D. Byrd, Eric C. Soller, Douglas L. Heintzelman M.D., Karen M. McNally-Heintzelman

Author Affiliations +

Proceedings Volume 4949, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII; (2003) https://doi.org/10.1117/12.476389
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

The use of indocyanine green-doped albumin protein solders has been shown to vastly improve the anastomotic strength that can be achieved by laser tissue repair techniques, while at the same time minimizing collateral thermal tissue damage. However, the safety of the degradation products of the chromophore following laser irradiation is uncertain. Therefore, we studied the feasibility of using alternative chromophores in terms of temperature rise at the solder/tissue interface, the extent of thermal damage in the sourrounding tissue, and the tensile strength of repairs. Biodegradable polymer scaffolds of controlled porosity were fabricated with poly(L-lactic-co-glycolic acid), using a solvent-casting and particulate-leaching technique. The porous scaffold acted as a carrier to the traditional protein solder composition of serum albumin and an absorbing chromophore mixed in deionized water. Two commonly used chromophores, indocyanine green and methylene blue were investigated, as well as blue and green food colorings. Temperature rise at the solder surface and at the interface between the solder and tissue were monitored by an IR temperature monitoring system and a type-K thermocouple, respectively, and the extent of thermal damage in the underlying tissue was determined using light microscopy. As expected, temperature rise at the solder/tissue interface, and consequently the degree of collateral thermal tissue damage, was directly related to the penetration depth of the laser light in the protein solder. Optimal tensile strength of repairs was achieved by selecting a chromophore concentration that resulted in a temperature of 66 ± 3°C at the solder/tissue interface.

Citation Download Citation

Grant T. Hoffman, Brian D. Byrd, Eric C. Soller, Douglas L. Heintzelman M.D., and Karen M. McNally-Heintzelman "Alternative chromophores for use in light-activated surgical adhesives: optimization of parameters for tensile strength and thermal damage profile", Proc. SPIE 4949, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII, (12 September 2003); https://doi.org/10.1117/12.476389

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available