Photothermal monitoring of interaction of carcinoma cells with cytostatic drugs in vitro

Dmitri Lapotko; Ehab Hanna; Martin Cannon

doi:10.1117/12.486316

19 June 2003 Photothermal monitoring of interaction of carcinoma cells with cytostatic drugs in vitro

Dmitri Lapotko, Ehab Hanna, Martin Cannon

Proceedings Volume 4962, Manipulation and Analysis of Biomolecules, Cells, and Tissues; (2003) https://doi.org/10.1117/12.486316
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Background/problem. Monitoring of tumor response to cancer chemotherapy and dose optimization for specific patients are the key factors for successful application of anti-tumor drugs. Using patient's tumor cells for preliminary in vitro drug screening may allow optimal selection of drug type and dose. Method. Single cell state was studied with photothermal microscope. Carcinoma cells were irradiated at 427 nm with 8 ns laser pulse with energy 30 - 40 μJ. Cell photothermal (PT) response amplitude and shape from each cell were analyzed and amount of cells that produced specific PT response was used as PT parameter. Parallel experiment included cell viability control. Results were obtained for two cytotoxic chemotherapy agents -- Platinol-aq and Adrucil. Incubation of cell suspensions for 90 min at 20 and 37°C caused changes in cell PT parameters. Reaction of carcinoma cells to the drug was very similar to reaction of hepatocytes to respiratory chain inhibition and reaction of RBC to osmotic pressure decrease. PT effect was found to be dose-dependent. PT method allows detecting drug-induced changes before cell death or morphological changes and therefore can be fast and sensitive modality for control of chemotherapy.

Citation Download Citation

Dmitri Lapotko, Ehab Hanna, and Martin Cannon "Photothermal monitoring of interaction of carcinoma cells with cytostatic drugs in vitro", Proc. SPIE 4962, Manipulation and Analysis of Biomolecules, Cells, and Tissues, (19 June 2003); https://doi.org/10.1117/12.486316

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