Multispectral fluorescence imaging techniques for nondestructive food safety inspection

Moon S. Kim; Alan M. Lefcourt; Yud-Ren Chen

doi:10.1117/12.516112

30 March 2004 Multispectral fluorescence imaging techniques for nondestructive food safety inspection

Moon S. Kim, Alan M. Lefcourt, Yud-Ren Chen

Proceedings Volume 5271, Monitoring Food Safety, Agriculture, and Plant Health; (2004) https://doi.org/10.1117/12.516112
Event: Optical Technologies for Industrial, Environmental, and Biological Sensing, 2003, Providence, RI, United States

Abstract

The use of spectral sensing has gained acceptance as a rapid means for nondestructive inspection of postharvest food produce. Current technologies generally use color or a single wavelength camera technology. The applicability and sensitivity of these techniques can be expanded through the use of multiple wavelengths. Reflectance in the Vis/NIR is the prevalent spectral technique. Fluorescence, compared to reflectance, is regarded as a more sensitive technique due to its dynamic responses to subtle changes in biological entities. Our laboratory has been exploring fluorescence as a potential means for detection of quality and wholesomeness of food products. Applications of fluorescence sensing require an understanding of the spectral characteristics emanating from constituents and potential contaminants. A number of factors affecting fluorescence emission characteristics are discussed. Because of relatively low fluorescence quantum yield from biological samples, a system with a powerful pulse light source such as a laser coupled with a gated detection device is used to harvest fluorescence, in the presence of ambient light. Several fluorescence sensor platforms developed in our laboratory, including hyperspectral imaging, and laser-induced fluorescence (LIF) and steady-state fluorescence imaging systems with multispectral capabilities are presented. We demonstrate the potential uses of recently developed fluorescence imaging platforms in food safety inspection of apples contaminated with animal feces.

Citation Download Citation

Moon S. Kim, Alan M. Lefcourt, and Yud-Ren Chen "Multispectral fluorescence imaging techniques for nondestructive food safety inspection", Proc. SPIE 5271, Monitoring Food Safety, Agriculture, and Plant Health, (30 March 2004); https://doi.org/10.1117/12.516112

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