Twisting biological objects by optical tweezers

Pal Ormos; Herbert van Amerongen; Sandor Bottka; Peter Galajda; Gyozo Garab; Huba Kirei; Laszlo Oroszi

doi:10.1117/12.558685

18 October 2004 Twisting biological objects by optical tweezers

Pal Ormos, Herbert van Amerongen, Sandor Bottka, Peter Galajda, Gyozo Garab, Huba Kirei, Laszlo Oroszi

Proceedings Volume 5514, Optical Trapping and Optical Micromanipulation; (2004) https://doi.org/10.1117/12.558685
Event: Optical Science and Technology, the SPIE 49th Annual Meeting, 2004, Denver, Colorado, United States

Abstract

We describe a novel method by which it is possible to apply and measure torque directly on particles grabbed in optical tweezers. It can be used to orient particles of micron size or even on single molecules, biopolymers by the use of test particles. The procedure is based on the observation that flat objects are oriented in an optical trap formed by linearly polarized light. The orienting torque originates from the anisotropic scattering of polarized light by the trapped particle. The phenomenon is characterized in detail, the physics is analysed. A tool is developed that exploits this effect to manuipulate biological macromolecules. Microscopic particles are produced by photopolymerisation that exploit this orientation effect and by which the torque is applied upon the biological object. In our system the applied torque can be turned on and off, it is controlled independently of the grabbing force of the tweezers during the manipulation process. The capabilities of the method are demonstrated. The method has great promise for application on DNA, DNA-protein complexes, actin filaments and other biopolymers.

Citation Download Citation

Pal Ormos, Herbert van Amerongen, Sandor Bottka, Peter Galajda, Gyozo Garab, Huba Kirei, and Laszlo Oroszi "Twisting biological objects by optical tweezers", Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); https://doi.org/10.1117/12.558685

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