Miniature biochip system for bioenvironmental applications

Musundi B. Wabuyele; Fei Yan; M. E. Martin; Guy D. Griffin; David L. Stokes; Dimitra N. Stratis-Cullum; Joel Mobley; Arpad A. Vass; Tuan Vo-Dinh

doi:10.1117/12.580126

7 December 2004 Miniature biochip system for bioenvironmental applications

Musundi B. Wabuyele, Fei Yan, M. E. Martin, Guy D. Griffin, David L. Stokes, Dimitra N. Stratis-Cullum, Joel Mobley, Arpad A. Vass, Tuan Vo-Dinh

Author Affiliations +

Proceedings Volume 5586, Advanced Environmental, Chemical, and Biological Sensing Technologies II; (2004) https://doi.org/10.1117/12.580126
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

An integrated multi-functional biochip based on integrated circuit complementary metal oxide semiconductor (CMOS) sensor array for use in medical diagnostics and pathogen detection has been described. The usefulness and potential of the biochip as a rapid, inexpensive screening tool for detection of bioenvironmental pathogens will be demonstrated. Detection of aerosolized spores was achieved by coupling the miniature system to a portable bioaerosol sampler, and the performance of the antibody-based recognition and enzyme amplification method was evaluated. The bioassay performance was found to be compatible with the air sampling device, and the enzymatic amplification was found to be an attractive amplification method for detection of low spore concentrations. The combined portable bioaerosol sampler and miniature biochip system detected 100 B. globigii spores, corresponding to 17 aerosolized spores/L of air.

Citation Download Citation

Musundi B. Wabuyele, Fei Yan, M. E. Martin, Guy D. Griffin, David L. Stokes, Dimitra N. Stratis-Cullum, Joel Mobley, Arpad A. Vass, and Tuan Vo-Dinh "Miniature biochip system for bioenvironmental applications", Proc. SPIE 5586, Advanced Environmental, Chemical, and Biological Sensing Technologies II, (7 December 2004); https://doi.org/10.1117/12.580126

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