Detection of biomolecules in complex media using surface plasmon resonance sensors

Michael R. Malone; Jean-Francois Masson; Margaret Barhnart; Stephen Beaudoin; Karl S. Booksh

doi:10.1117/12.630736

11 November 2005 Detection of biomolecules in complex media using surface plasmon resonance sensors

Michael R. Malone, Jean-Francois Masson, Margaret Barhnart, Stephen Beaudoin, Karl S. Booksh

Author Affiliations +

Proceedings Volume 6007, Smart Medical and Biomedical Sensor Technology III; 60070V (2005) https://doi.org/10.1117/12.630736
Event: Optics East 2005, 2005, Boston, MA, United States

Abstract

Detection of multiple biologically relevant molecules was accomplished at sub-ng/mL levels in highly fouling media using fiber- optic based surface plasmon resonance sensors. Myocardial infarction markers, myoglobin and cTnI, were quantified in full serum with limits of detection below 1 ng/mL. Biologically relevant levels are between 15-30 ng/mL and 1-5 ng/mL for myoglobin and cTnI respectively. Cytokines involved in chronic wound healing, Interleukin 1, Interleukin 6, and tumor necrosis factor α, were detected at around 1 ng/mL in cell culture media. Preliminary results in monitoring these cytokines in cell cultures expressing the cytokines were obtained. The protein diagnostic of spinal muscular atrophy, survival motor neuron protein, was quantified from cell lysate. To obtain such results in complex media, the sensor's stability to non-specific protein adsorption had to be optimized. A layer of the N-hydroxysuccinimide ester of 16-mercaptohexadecanoic acid is attached to the sensor. This layer optimizes the antibody attachment to the sensor while minimizing the non-specific signal from serum proteins.

Citation Download Citation

Michael R. Malone, Jean-Francois Masson, Margaret Barhnart, Stephen Beaudoin, and Karl S. Booksh "Detection of biomolecules in complex media using surface plasmon resonance sensors", Proc. SPIE 6007, Smart Medical and Biomedical Sensor Technology III, 60070V (11 November 2005); https://doi.org/10.1117/12.630736

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE