Four-channel optical detection on protein-patterned bioCD

Xuefeng Wang; Ming Zhao; David D. Nolte

doi:10.1117/12.701236

10 February 2007 Four-channel optical detection on protein-patterned bioCD

Xuefeng Wang, Ming Zhao, David D. Nolte

Proceedings Volume 6447, Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV; 64470L (2007) https://doi.org/10.1117/12.701236
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We have developed a four-channel detection method on a protein-patterned BioCD that simultaneously measures fluorescence, Rayleigh scattering and/or diffraction, and two interferometric channels in orthogonal quadratures: one that measures differential phase and the other that measures direct phase. The latter two channels constitute label-free interferometric protein detection, while fluorescence and Mie scattering detection provide complementary tools. The BioCD is constructed as dielectric coated disks. Protein molecules patterned on dielectric films change the Fresnel reflection coefficient of the films. The change is exhibited in two ways: the reflection coefficient and the phase are both modulated. These are detected simultaneously by a split detector and designated as "amplitude" signal and "phase contrast" (PC) signal. We are able to scan and image patterned proteins across an entire coated disk with high speed in four channels. A single-analyte immunoassay shows strong correlation between the fluorescence channel and the interferometry channel with a detection limit of 10 ng/ml in a complex protein background (rat lysate) concentration of 7 mg/ml.

Citation Download Citation

Xuefeng Wang, Ming Zhao, and David D. Nolte "Four-channel optical detection on protein-patterned bioCD", Proc. SPIE 6447, Nanoscale Imaging, Spectroscopy, Sensing, and Actuation for Biomedical Applications IV, 64470L (10 February 2007); https://doi.org/10.1117/12.701236

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