Refractive index determination by index-mismatch-induced spherical aberration

Ping-Jung Su; Peter T. Fwu; Vladimir Hovhannisyan; Chen-Yuan Dong

doi:10.1117/12.728387

16 July 2007 Refractive index determination by index-mismatch-induced spherical aberration

Ping-Jung Su, Peter T. Fwu, Vladimir Hovhannisyan, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III; 66300C (2007) https://doi.org/10.1117/12.728387
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Two-photon microscopy has been successfully applied in biological and material sciences since 1990. However, it is known that the resolution of two-photon imaging is adversely affected from the index mismatch induced spherical aberrations. Spherical aberration increases the focal volume and causes degradation of image resolution as one images deeper into the specimen. In this work, we propose to use this intrinsic artifact to measure the refractive index in specimens of uniform refractive indices. Using the intensity profiles of standard refractive liquids as our reference, we can compare the intensity profile of an unknown specimen of uniform refractive index with that of the reference to determine the refractive index of the unknown specimen.

Citation Download Citation

Ping-Jung Su, Peter T. Fwu, Vladimir Hovhannisyan, and Chen-Yuan Dong "Refractive index determination by index-mismatch-induced spherical aberration", Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300C (16 July 2007); https://doi.org/10.1117/12.728387

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