Effects of surfactants on the formation of microdroplets in the flow focusing microfluidic device

Nan Wu; Y. Zhu; P. W. Leech; B. A. Sexton; S. Brown; C. Easton

doi:10.1117/12.769326

27 December 2007 Effects of surfactants on the formation of microdroplets in the flow focusing microfluidic device

Nan Wu, Y. Zhu, P. W. Leech, B. A. Sexton, S. Brown, C. Easton

Proceedings Volume 6799, BioMEMS and Nanotechnology III; 67990C (2007) https://doi.org/10.1117/12.769326
Event: SPIE Microelectronics, MEMS, and Nanotechnology, 2007, Canberra, ACT, Australia

Abstract

Droplet-based in vitro compartmentalization (IVC) platform is a powerful tool in protein analysis. Reliable formation of microdroplets is important for the development of the microfluidic chip. In this study, we will examine the effect of surfactants on the formation of microdroplets in the flow focusing microfluidic device which is needed for enzyme evolution. Surfactants of Span 80 and Tween 20 are used in the continuous and dispersed phases, respectively. The droplet formation was affected distinguishably with the presence of Span 80 and Tween 20. The size of droplets decreased as the concentration of Span 80 increased. And the decrease was more pronounced with the combination of Span 80 and Tween 20.

Citation Download Citation

Nan Wu, Y. Zhu, P. W. Leech, B. A. Sexton, S. Brown, and C. Easton "Effects of surfactants on the formation of microdroplets in the flow focusing microfluidic device", Proc. SPIE 6799, BioMEMS and Nanotechnology III, 67990C (27 December 2007); https://doi.org/10.1117/12.769326

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